Transplantation of bcl-2 gene-modified bone marrow mesenchymal stem cells improves cardiac function and angiogenesis in rabbit ischemic car-diac insufficiency model

AIM: To investigate the effects of transplantation of bone marrow mesenchymal stem cells (BMSCs) modified by bcl-2 gene on myocardial cell apoptosis, angiogenesis and cardiac function in the rabbit after acute myocardial infarction (MI).METHODS: The rabbit BMSCs were isolated, cultured and purified in vitro. The BMSCs were transfected with adenovirus or adenovirus-Bcl-2. The rabbit model of MI was established by ligation of left anterior descending branch. The rabbits were injected with Ad-Bcl-2-BMSCs (MI+Bcl-2-BMSCs group), Ad-BMSCs (MI+BMSCs group) and DMEM (MI group) in infarction marginal zone 2 weeks after ligation. The cardiac function was evaluated by echocardiography.The apoptosis of myocardial cells was measured by TUNEL. The mRNA expression of VEGF was detected by real-time PCR. The expression of CD31 was examined by immunohistochemical staining, and new blood capillaries were counted at 4 weeks after BMSCs transplantation. The correlation of the above values with cardiac function was analyzed.RESULTS: The cardiac function was better, the apoptotic rate was lower, the mRNA expression of VEGF and the capillary density were higher in both MI+Bcl-2-BMSCs group and the MI+BMSCs group than those in MI group, and those in MI+Bcl-2-BMSCs group increased more obviously.The left ventricular ejection fraction (LVEF) had a negative correlation with the myocardial cell apoptosis rate. A positive correlation with the mRNA expression level of VEGF and the capillary density was also observed.CONCLUSION: The transplantation of BMSCs modified by bcl-2 gene significantly reduces the myocardial cell apoptosis, promotes angiogenesis, improves heart function of the rabbits with MI.     

豫西黄土高原区域苹果矮砧集约栽培技术规程

针对豫西黄土高原区域苹果矮砧集约栽培发展趋势,在分析该区域自然条件和生产实际间的基础上,依据国家苹果产业技术体系《苹果矮砧集约栽培模式技术规范》,从砧穗组合、苗木选择、栽植密度、架材选择安装、整形修剪及配套栽培措施等6个方面制定了豫西黄土高原区域苹果矮砧集约栽培技术规程,以期为苹果矮砧集约栽培提供科学技术依据。     

蔬菜根结线虫生防细菌的筛选及菌株HJT2-1种类的初步鉴定

以天津地区7个区县的蔬菜大棚土壤中分离出的306株细菌菌株为试材,采用亚甲基蓝染色法,研究306株细菌菌株发酵液对南方根结线虫2龄幼虫的影响。结果表明:细菌菌株HJT2-1发酵液对南方根结线虫2龄幼虫的校正死亡率较高为81.3%,其温室盆栽防效为55.6%,略低于阿维菌素防效。采用对细菌核糖体DNA的内转录间隔区进行分析的方法将菌株HJT2-1初步鉴定为假单胞菌属细菌。该试验获得了对根结线虫2龄幼虫具有较高致死率的细菌菌株HJT2-1,为开发防治根结线虫的生防制剂奠定了基础。     

丰帅苹果品种选育报告

丰帅是江苏省丰县农委果树研究所从金帅中选育的芽变品种,于2011年通过江苏省果茶花品种鉴定,鉴定证号为苏鉴果201109号。该品种在保留金帅优良特性的基础上,具有果实高桩、果面光洁、果锈较少的特点,适合在黄河故道区域发展。     

具有广阔发展前景的几种云南天然香料

叙述了云南省天然香料资源开发利用简况及生产现状，提出了发展香料产业区域布局的设想。重点论述了香荚兰、肉桂、薄荷的国内外生产动向及在云南的发展现状和前景。     

人为干扰对额济纳胡杨林群落组成及更新的影响

额济纳绿洲是我国西北干旱地区的一道强大的生态防线。但由于水资源匮乏和人为干扰影响,绿洲中胡杨林已严重退化。我们认为人为干扰会对胡杨群落的组成和更新造成影响。应用双向指示种分析法(TWINSPAN)和典范对应分析(CCA),从人为干扰角度对额济纳不同地区胡杨群落进行了分类和排序。结果表明:人为干扰会影响群落林下物种多样性和根孽幼苗生长情况;林下物种多样性主要受旅游干扰影响,并与总干扰强度的关系遵循"中度干扰假说";根孽幼苗生长情况与放牧干扰呈负相关,当根孽幼苗数多的时候,一定程度上能够抵御外界干扰对其生长状况的影响;适度的人为干扰可以增加物种多样性,但要保证胡杨林的繁殖更新,需要以消除放牧干扰影响为前提。     

珍稀树种小叶银缕梅和宝华玉兰自然现状及其就地保护研究

野外实地分别调查了江苏宜兴市和句容市分布的珍稀树种小叶银缕梅（Parrotia subaequalis）和宝华玉兰（Magnolia zenii Cheng），掌握了这2种珍稀树种在江苏的自然分布状况及其群落学特征，统计了它们的种群规模和种群结构。经综合分析，对其就地保护的现状及保护建议提出了看法。     

红松高生长模型的研究(英文)

高生长模型是定量研究树木生长过程的有效途径,本文应用双曲线方程、Logistic方程、三个参数的Richards方程及四个参数的Richards方程对红松的高生长进行了研究。结果表明四个参数的Richards方程在模拟红松高生长时最为合适,且当其参数取不同的数值时,该方程可转化为其它理论方程。在使用四个参数的Richards方程时,可根据立地条件事先给定树高最大值,这样可使模型更符合实际情况。此外,文中还对一种以固定年龄时实际树高为参数值的模型进行了讨论,该模型对于计算给定树木的高生长将更为有效。     

落叶松生长率测定方法的探讨

落叶松生长率是落叶松资源档案管理的重要资料，其测定方法较多，笔者认为，用复利式生长率比用普雷斯勒式生长率更接近实际值，可达到要求的精度，并可保持计算的系统性。笔者还认为，区分立地条件测定龄级带皮材积复利生长率的方法，比区分立地条件测定龄组去皮材积生长率的方法更科学、实用性更强、使用范围更广，前者可为基地化、规模化栽培落叶松速生丰产林提供科学依据。     

Cellular and ultrastructural changes in the seedling roots of upland rice (Oryza sativa) under the stress of two allelochemicals from Ageratina adenophora

Two sesquiterpene‐derivative compounds, 4,7‐dimethyl‐1‐(propan?2‐ylidene)–1,4,4a,8a‐tetrahydronaphthalene‐2,6(1H, 7H)‐dione (DTD) and 6‐hydroxy‐5‐isopropyl‐3,8‐dimethyl?4a,5,6,7,8,8a‐hexahydronaphthalen‐2(1H)–one (HHO), are the major putative allelochemicals of the aqueous leachates of Ageratina adenophora. A laboratory experiment was conducted, using the hydroponic method, to evaluate the cellular and ultrastructural changes in the seedling roots of upland rice under the stress of DTD and HHO. The subsequent changes were observed in the treated upland rice roots in comparison with their controls. The scanning electron microscopy results showed that the DTD‐treated root tip cells turned into an irregular arrangement and shape and that most of them were wizened, with a poor cytoplasm. In the HHO treatment, the root tips had many irregularly shaped cells, with a greater number of sloughing cells, as well as short, wide cells that resulted in spherical and wider, but shorter, roots. At the ultrastructural level, DTD and HHO induced irregularly shaped and lobed nuclei, increased cytoplasmic vacuolation, reduced ribosome density and dictyosomes, and a reduced number of mitochondria in the cells, which indicated limited protein transportation and a reduced capability to export substances for cell development and growth in the upland rice seedling roots. The overall effect of HHO on the upland rice seedlings was more pronounced than that of DTD.